Composition for nucleic acid sequencing

专利名称：Composition for nucleic acid sequencing发明人：Jonas Korlach,Watt W. Webb,Michael

Levene,Stephen Turner,Harold G.Craighead,Mathieu Foquet

申请号：US11285422申请日：20051121公开号：US07416844B2公开日：20080826

摘要：The present invention is directed to a method of sequencing a target nucleicacid molecule having a plurality of bases. In its principle, the temporal order of baseadditions during the polymerization reaction is measured on a molecule of nucleic acid,i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acidmolecule to be sequenced is followed in real time. The sequence is deduced byidentifying which base is being incorporated into the growing complementary strand ofthe target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme ateach step in the sequence of base additions. A polymerase on the target nucleic acidmolecule complex is provided in a position suitable to move along the target nucleic acidmolecule and extend the oligonucleotide primer at an active site. A plurality of labelledtypes of nucleotide analogs are provided proximate to the active site, with each

distinguishable type of nucleotide analog being complementary to a different nucleotidein the target nucleic acid sequence. The growing nucleic acid strand is extended by usingthe polymerase to add a nucleotide analog to the nucleic acid strand at the active site,where the nucleotide analog being added is complementary to the nucleotide of the

target nucleic acid at the active site. The nucleotide analog added to the oligonucleotideprimer as a result of the polymerizing step is identified. The steps of providing labellednucleotide analogs, polymerizing the growing nucleic acid strand, and identifying theadded nucleotide analog are repeated so that the nucleic acid strand is further extendedand the sequence of the target nucleic acid is determined.

申请人：Jonas Korlach,Watt W. Webb,Michael Levene,Stephen Turner,Harold G.Craighead,Mathieu Foquet

地址：Ithaca NY US,Ithaca NY US,Ithaca NY US,Ithaca NY US,Ithaca NY US,Ithaca NY US

国籍：US,US,US,US,US,US

代理机构：Wilson Sonsini Goodrich & Rosati

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